Effect of microwave-assisted enzymatic hydrolysis of cricket (Gryllodes sigillatus) protein on ACE and DPP-IV inhibition and tropomyosin-IgG binding

Effect of microwave-assisted enzymatic hydrolysis of cricket (Gryllodes sigillatus) protein on ACE and DPP-IV inhibition and tropomyosin-IgG binding

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Techniques, such as microwave-assisted enzymatic hydrolysis, provide options to generate insect-bioactive peptides.However, allergenicity of these novel bioactive peptides remains a concern.Cricket protein was enzymatically hydrolyzed using conventional heating or microwave radiation.

Protein hydrolysates were evaluated for DPP-IV and ACE inhibition activity, and tropomyosin-IgG Wood Print reactivity (a major cricket allergen).ACE and DPP-IV inhibition was highest in the microwave-hydrolyzed protein (IC50 = 0.096 mg/ml and 0.

27 mg/ml, respectively).All samples displayed tropomyosin-IgG reactivity; however, the lowest binding was obtained with the microwave-hydrolyzed protein.Raman spectroscopy revealed conformational changes, particularly in the Amide I and S-S regions, which may correlate to the observations in the immunochemical reactivity.

In conclusion, microwave-assisted enzymatic hydrolysis can be a useful method for generating bioactive Ski de fond - Femme - Sous-vetements - Synthetique peptides from insect proteins and lowering their immunoreactivity, validating the potential of these treatments to generate bioactive, hypoallergenic peptides for food and pharmaceutical applications.